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Molecular Dynamics Inc laser computer-assisted densitometry
Laser Computer Assisted Densitometry, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/laser+computer-assisted+densitometry/laser+computer+assisted+densitometry/pm22178948-56-9-12
Average 90 stars, based on 1 article reviews
laser computer-assisted densitometry - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Differences in the response of UCP1 mRNA to hormonal stimulation between rat and mouse primary cultures of brown adipocytes.
Article Snippet: Autoradiograms were obtained from the filters and quantified by laser computer-assisted densitometry (Molecular Dynamics).

Article Title: Potent thermogenic action of triiodothyroacetic acid in brown adipocytes.
Article Snippet: Triiodothyroacetic acid (TRIAC) is a triiodothyronine (T3) metabolite with high affinity for T3 nuclear receptors.. We compared the thermogenic action of TRIAC versus T3 in brown adipocytes, by studying target genes known to mediate thermogenic action: uncoupling protein 1 (UCP-1), a marker of brown adipocytes, and type II-5¢deiodinase (D2), which provides the T3 required for thermogenesis.. TRIAC is 10–50 times more potent than T3 at increasing the adrenergic induction of UCP-1 mRNA and D2 activities.

Article Title: Triiodothyronine is required for the stimulation of type II 5'-deiodinase mRNA in rat brown adipocytes.
Article Snippet: Martinez-deMena, Raquel, Arturo Hernández, and Maria-Jesús Obregón.. Triiodothyronine is required for the stimulation of type II 5 -deiodinase mRNA in rat brown adipocytes.. Am J Physiol Endocrinol Metab 282: E1119–E1127, 2002; 10.1152/ajpendo.00433.2001.—Type II 5 -iodothyronine deiodinase (D2), produces triiodothyronine (T3) and is stimulated by cold exposure via norepinephrine (NE) release in brown adipose tissue.

Article Title: Presence and mRNA expression of T3 receptors in differentiating rat brown adipocytes.
Article Snippet: Nuclear T3 binding and T3 receptors (TR) expression were studied in brown adipocytes differentiated in culture from precursor cells.. High affinity T3 receptors were found.. During adipocyte differentiation maximal binding capacity (MBC) was doubled (up to 763 fmol T3/mg DNA), and an apparent decrease in receptor affinity was also observed (due to a decrease in the association rate constant).

Article Title: Gene expression from the imprinted Dio3 locus is associated with cell proliferation of cultured brown adipocytes.
Article Snippet: Filters were hybridized for 20 h at 50 C [40% formamide, 5 saline sodium citrate (SSC), 2 Denhardt’s, 0.1% sodium dodecyl sulfate (SDS)], washed four times in 2 SSC per 0.2% SDS at room temperature for 15 min and then twice in 0.1 SSC per 0.2% SDS at 65 C for 20 min. Autoradiograms were obtained from the filters and quantified by laser computer-assisted densitometry (Molecular Dynamics, Sunnyvale, CA).

Article Title: Transcriptional activation of type III inner ring deiodinase by growth factors in cultured rat brown adipocytes.
Article Snippet: Filters were hybridized for 20 h at 50 C [40% formamide, 5 3 SSC (standard saline citrate), 2 3 Denhardt’s, and 0.1% SDS] and washed four times in 2 3 SSC-0.2% SDS at room temperature for 15 min, then twice in 0.1 3 SSC-0.2% SDS at 65 C for 20 min. Autoradiograms were obtained from the filters and quantified by laser computer-assisted densitometry (Molecular Dynamics, Sunnyvale, CA).



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